Sensitive fluorescence detection of SARS-CoV-2 RNA in clinical samples via one-pot isothermal ligation and transcription
Woo CH, Jang S, Shin G, Jung GY, Lee JW. Nat Biomed Eng. 2020 Sep 18:1-12. doi: 10.1038/s41551-020-00617-5. Pubmed
Massively parallel kinetic profiling of natural and engineered CRISPR nucleases
Jones SK Jr, Hawkins JA, Johnson NV, Jung C, Hu K, Rybarski JR, Chen JS, Doudna JA, Press WH, Finkelstein IJ. Nat Biotechnol. 2020 Sep 7. doi: 10.1038/s41587-020-0646-5. Pubmed
Systematic in vitro profiling of off-target affinity, cleavage and efficiency for CRISPR enzymes
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Splice-Switching Antisense Oligonucleotides Reduce LRRK2 Kinase Activity in Human LRRK2 Transgenic Mice
Korecka JA, Thomas R, Hinrich AJ, Moskites AM, Macbain ZK, Hallett PJ, Isacson O, Hastings ML. Mol Ther Nucleic Acids. 2020 Sep 4;21:623-635. Pubmed
QR-313, an antisense oligonucleotide, shows therapeutic efficacy for treatment of dominant and recessive dystrophic epidermolysis bullosa: a preclinical study
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Extracellular microRNAs in human circulation are associated with miRISC complexes that are accessible to anti-AGO2 antibody and can bind target mimic oligonucleotides
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A mouse tissue atlas of small noncoding RNA.
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Knockdown of liver-derived factor XII by GalNAc-siRNA ALN-F12 prevents thrombosis in mice without impacting hemostatic function
Liu J, Cooley BC, Akinc A, Butler J, Borodovsky A. Thromb Res. 2020 Aug 28;196:200-205. Pubmed
2′-O-Methyl at 20-mer Guide Strand 3′ Termini May Negatively Affect Target Silencing Activity of Fully Chemically Modified siRNA
Davis SM, Sousa J, Vangjeli L, Hassler MR, Echeverria D, Knox E, Turanov AA, Alterman JF, Khvorova A. Mol Ther Nucleic Acids. 2020 Sep 4;21:266-277. Pubmed
Cell Type Impacts Accessibility of mRNA to Silencing by RNA Interference
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